Chitosan-alginate Microfibers Generation on a Microfluidic Chip and Encapsulation of Hepg2 Cells
نویسندگان
چکیده
The successful encapsulation of human hepatocellular carcinoma (HepG2) cells would assist applications in tissue engineering. In this paper, we have developed a method to produce chitosan-alginate microfibers within a microfluidic chip. Also, by suspending cells in the mixing solution, we fabricated HepG2 cell-laden fibers. The encapsulated HepG2 cells in the chitosan–alginate fibers were more viable than cells encapsulated in pure alginate fibers, suggesting that chitosan provides a better environment for HepG2 cells than alginate. The proposed technique provided an uniform distribution of cells within the fibers, which has been a critical yet previously unmet requirement for tissue engineering.
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